Thursday, January 30, 2020
Introduction to Prokaryotes Essay Example for Free
Introduction to Prokaryotes Essay Prokaryotes are single-celled organisms that can survive in extreme environments. Bacteria is the more numerous type of prokaryotes. The group hypothesizes that the samples taken from different environments will all cultivate diverse morphology in fast growing rates in each environment. The aseptic technique was used to cultivate bacteria from different environments. The diversity of morphology and the growing rate of the bacteria was different in each environment. Introduction Prokaryotes are the oldest known life-forms, having existed for the last 3. 5 billion years. Microscopic in size, they are single-celled organisms. Prokaryotic species can survive in extreme habitats that the other life-forms are not capable of inhabiting. Prokaryotes have different shapes, the three most common shapes are spherical (cocci), rod shaped (bacilli), and spiral (spirilla). The prokaryotic cellular structures are unique to their classification. Prokaryotes have an external cell wall and a plasma membrane. The cell wall keeps the shape of the cell, protects the cell, and averts the cells from bursting in a hyposmotic environment. Prokaryotic cells contain a unique material called peptidoglycan (Sadava et al. , 2011). See more: how to write an introduction Also metabolic diversity is among the criteria used in classifying prokaryotes. The term nutrition refers to the means an organism uses to obtain two energy sources: energy and a carbon source. Carbon sources may be either organic, meaning from a living organism, or inorganic, such as carbon dioxide. Prokaryotes split into two lineages known as Archae and Bacteria. The Bacteria are more numerous than the Archae. Bacteria can be endospore-forming bacteria. Bacteria that form endospores are able to survive harsh and severe conditions. Bacteria can also be Enteric Bacteria, they inhabit the intestinal tracts of animals. One species is Escheria coli. Wild-type Escheria strains are able to grow on a variety of carbon and energy sources, such as sugars and amino acids. Some strains of Escheria are pathogenic. The detection of Escheria coli in water is a sign of contamination. Another group of pathogenic enteric bacteria are members of the genus Salmonella. These members are responsible for food poisoning and typhoid. Prokaryotes play very important roles in our environment. They are involved in the cycling of nutrients and elements in a variety of ways. Many prokaryotes are decomposers that metabolize organic compounds in dead organisms. These decompositions processes result in the return of vast quantities of carbon dioxide, inorganic nitrogen, and sulfur to our ecosystems. Other species are important as symbiotic partners with other organisms (Walsh et. al. , 2010). The diversity of the prokaryotic world is huge, and to have a better sense of knowledge of bacteria diversity in different environments an experiment to observe bacteria growth diversity in colder temperature is conducted. The group hypothesizes that the samples taken from different environments will all cultivate diverse morphology in fast growing rates in each environment. The independent variable in the experiment is the temperature control and the dependent variable is the number of colonies. Materials and Methods Seven different environments were chosen to create bacteria from and cultivated on a nutrient-rich media in eight Petri dishes. The bacteria are cultivated on TSA medium, an all-purpose medium used for cultivating all types of bacteria. Sterile water and sterile swabs are used to sample the bacteria from the environment. To make sure that the bacteria was loosened from the environment and stuck on to the swab, the swab was dipped in the sterile water immediately before taking the sample. Carefully opened the Petri dish and swiped the swab across the plate in a â€Å"Z†pattern. Closed the Petri dish and marked it with its corresponding environment. This was repeated seven times each with a different environment. The first environment was the frame of the classroom chalkboard. The second environment was the chair seat of the classroom. The third environment was the bottom of the shoe of one of our group members. The fourth environment was the floor mat inside the doorway of the Biology building. The fifth nvironment was the stair railing handle from the stairwell of the Biology building. The sixth environment was the spacebar on the keyboard of the laboratory computer. The seventh environment was the mouthpiece of the water fountain in the Biology building. To enable us to check whether or not our aseptic technique was effective the eight Petri dish was our control plate that was struck with the sterile water only. These streaks with sterile water represent control treatments. The bacteria was incubated at 37 °C for 2-3 days and then put into the refrigerator for storage. Results Two of the Petri dishes had small bacteria diversity and also a slow growth rate- the chair seat of laboratory environment sample and the water fountain mouthpiece sample (Table 1). Three of the Petri dishes had medium bacteria diversity and regular growth- the frame of the chalkboard, the stair railing handle from the stairwell, and the spacebar of the keyboard (Table 1). The other two Petri dishes had medium bacteria diversity and fast growth rate- the bottom of the shoe and the floor mat inside the doorway of the Biology building (Table 1). The Petri dish with the sterile water streaks had no bacteria growth or diversity indicating our aseptic technique was effective. Discussion The results that were obtained in the experiment did not support the hypothesis that there would be large diversity and fast growing rates in each environment. Every environment sample had its own growth rate and bacteria diversity. The primary reason may be that conditions are rarely optimum. Scientists who study bacteria try to create the optimum environment in the lab: culture medium with the necessary energy source, nutrients, pH, and temperature, in which bacteria grow predictably. Most of the strains used in the classroom either require oxygen for growth or grow better with oxygen. These bacteria will grow better on agar plates, where air readily diffuses into the bacterial colony, or in liquid cultures that are shaken. Since diffusion of oxygen into liquid depends on the surface area, it is important to have a large surface; volume ratio. This means that cultures will grow best in flasks in which the volume of liquid is small relative to the size of the vessel. Also another factor that affects growth is the nutritional medium. Bacteria grow best when optimal amounts of nutrients are provided.
Wednesday, January 22, 2020
Macbeth :: essays research papers
The Elizabethan Concept of the King Intro: Our actions define who we are. Who we are, defines our actions. This concept has been common to man throughout history, and the Elizabethans are no exception. They applied this statement to their kings in an attempt to classify them as good or bad, strong or weak. In the play Macbeth, we notice many references to the characteristics of a good king. The Elizabethan view was that if a king was weak, he would be taken advantage of and killed. Thesis Statement: The Elizabethan view of the king has taught us that a king must possess certain characteristics in order to survive. A king must have physical and emotional strength, good judgement, intelligence, confidence, and rational thinking, or he will be killed. 1st point - physical and emotional strength Topic Sentence: A good king must possess physical and emotional strength or he will be taken advantage of and killed. Duncan -     Act I Scene II -     Reveals King Duncan as mild and benevolent, but weak and unfit to be a king. -     Shows his physical weakness because there was rebellion and invasion, but Duncan was not out leading his army. (I, II, 1) -     Act I Scene IV -     Shows Duncan’s emotional weakness -     He is too weak and shows over-gratitude. (I, IV, 33) Macbeth -     Macbeth shows emotional weakness when he hallucinates -     During Macbeth’s soliloquy in Act II Scene I, he hallucinates and sees a dagger floating in front of him. (II, I, 33) As you can see through these examples, both Duncan and Macbeth show physical weakness and in return are both killed. 2nd point - good judgement Topic Sentence: In order for a king to stay in power and stay alive, he must make the right decisions and use good judgement. Duncan -     Uses bad judgement by giving Macbeth the title of Thane of Cawdor -     Rewards Macbeth and not Banquo which could cause envy in Banquo and cause Macbeth to feel more important than Banquo -     Shows that he is a poor judge of human nature (I, IV, 11) -     Shows poor judgement in choosing a time to reveal Malcolm as his successor to the throne -     Could not see Macbeth’s ambition which others could see (I, III, 120) -     Uses bad judgement when deciding to sleep at Macbeth’s house (I, VI, 1) Macbeth -     Makes a bad decision to kill Banquo which made the people more suspicious -     Decides to kill Macduff’s family, which gave Macduff the motive to kill Macbeth. (IV, III, 233) 3rd point - intelligence, confidence, and rational thinking Topic Sentence: To avoid being killed, a king must be intelligent, rational, and confident.
Monday, January 13, 2020
Decoding the PMP?® Exam
PM Introduction Since the early asses, project managers have prepared for the Project Management Professional ([email protected]) Exam through intense study of the Project Management Institute's ([email protected]) A Guide to the Project Management Body of Knowledge ([email protected] Guide). Throw in a few ancillary primers and then take practice exam after practice exam and you've got a good chance of passing.What this approach lacks is an intuitive understanding of what it takes to put the [email protected] Guide into practice. Many fresh-faced Amps can experience inefficiencies and extra challenges early on, until they overcome this handicap with on-the-Job experience. Depending on the scope and objectives of your project, this could have business-wide ramifications. Why learn from your mistakes when you can prevent them? The Task-Skill Method†* of PM Exam preparation offers a new approach that is more effective and offers more benefits than Just getting you rea dy to pass the exam.The Task-Skill Method prepares candidates through intensive review of the expected roles and responsibilities of a Certified Project Management Professional as identified in Mi's PM Role Delineations Study (REDS), also known as the PM Exam Blueprint. Armed with a fundamental understanding of on-the-Job requirements, you will better understand PM Exam questions and achieve higher overall scores. What's more, you'll be ready to immediately have an impact on your projects.The Task-Skill Method Revealed The Task-Skill Method is the result of more than a decade of lessons learned and process improvement. The breakthrough change lies in the foundational approach. While traditional PM Exam prep techniques prepare you to pass by essentially caching to the exam,' the new Task-Skill Method prepares you by teaching to the role of a PM The . ‘ Task-Skill Method still incorporates significant MAMBO Guide study, exam-taking skills and practice exams. However, they are us ed as reference standards and study or practice aids, rather than foundational requisites.While most traditional approaches are built around the framework of Knowledge Areas, Process Groups and Process Inputs, Tools and Techniques and Outputs, the new Task-Skill Method is built around the framework of the 37 individual Tasks and 65 individual PM Knowledge/Skill Sets of the PM Exam Blueprint. These are well defined and generally form the roles and responsibilities of a PM After . Taking into consideration overlap and redundancy, the Task-Skill Method boils down to 28 individual Tasks and 28 individual Knowledge/Skill Sets.What's more, these Tasks and Knowledge/Skill Sets serve as the foundation for all questions on the PM Exam, virtually eliminating any surprises. The 2012 PM pulse of the Profession study found that organizations with more than 35% PM certified project managers had better project performance. Keeping It Simple The Task-Skill Method reduces the amount of information y ou need to consume, sousing your objectives on what matters most and speeding up prep time. With traditional approaches, you can study up to 100 hours or more, but with the new Task-Skill Method, preparation time can be decreased by at least 25%.In a facilitated classroom environment, preparation time can require as little as 35-40 hours. Forty percent of your preparation time should be focused on the 28 PM Tasks and another 40% on the 28 PM Knowledge/Skill Sets. All should be clearly identified, understood, studied and sufficiently mastered. You should focus on understanding when and where each Task is typically performed across the project lifestyle and here each Skill Set is most essential. The remaining 20% of your time should be devoted to PM practice exams. This will hone your test-taking skills and serve as an additional learning resource.For example, in a 60-hour, self-paced, self-study plan, 12 hours would be devoted to Practice Exams, 24 hours to PM Tasks and 24 hours to P M Knowledge/Skill Sets. A Quick Look at the Exam The PM Certification Exam is a demonstration of your suitability to function as a Certified Project Management Professional. The exam includes 200 questions, many of them situational. Your score reflects your ability to: correctly identify recognized best practices; 2 use proper terminology; and 3 use logical reasoning to apply appropriate tools and techniques in given situations.PM Practice Exams 28 PM Tasks Knowledge/ Skill sets To ensure you are truly deserving of the PM Credential, many questions are deliberately made extra challenging. If you pass, you are awarded the PM Credential. If not, you can retake the exam up to three times in a one-year period. The Tasks and Skill Sets identified in the Task-Skill Method serve as the foundation for all questions on the PM Exam. Must-Know PM Tasks As a PM you may be expected to perform en or all of these 28 identified PM Tasks at various times during your project management career.The PM Exam will thoroughly test your understanding of each one by requiring you to demonstrate adequate knowledge of recognized best practices with respect to each Task. The PM Exam Blueprint identifies 37 individual PM Tasks. With many Tasks, there is overlap and some redundancy. For organization, efficiency and practical study purposes, the new Task-Skill Method effectively consolidates the 37 Blueprint Tasks to 28. Following is a detailed list of each must-know PM Task and the corresponding study aids. Evaluate Project Feasibility It is essential to establish a high-level understanding of the project early on.Is the project high risk, low risk, straightforward, complex? What constraints and assumptions are in consideration? How will you structure the project life cycle? What strategies will work? Study: [email protected] Guide sections 3. 3 and 4. 1 Perform a Stakeholder Analysis Managing stakeholder expectations is considered the top priority each and every day by many Amps. Study: [email protected] Guide section 10. 1 3 Develop a Project Charter A Project Charter is the formal document intended to authorize a project (or project phase).It briefly describes high-level project characteristics and identifies/authorizes the project manager. Study: [email protected] Guide section 3. 3 and 4. 1 4 Define Project Deliverables It is essential to get a firm understanding of the project scope-?what the project is and what it isn't-?early in project planning. Study: [email protected] Guide sections 5. 1 and 5. 2 5 Create a Work Breakdown Structure Once scope has been determined and agreed upon among key stakeholders, it should be broken down into manageable pieces.
Sunday, January 5, 2020
State Versus National Standards
As you write lesson plans, you will need to refer to standards for your subject area. Standards are created to ensure that students from one classroom to another are taught the same basic information in a particular subject. While that concept might seem simple stated as such, it can, in fact, be much more complicated for the individual classroom teacher. State Standards The situation is further complicated by the periodic changes that occur to standards. When a particular curriculum area meets to change their standards, teachers are handed and expected to teach to a new group of standards from that point on. This can cause problems when drastic changes occur and teachers are still using textbooks based on the older standards. So why does this situation exist? The answer lies in flexibility and the desire for local control. States are able to determine what is important for their citizens and focus the curriculum accordingly. National Standards Will there ever be mandated national standards? At this time, it looks doubtful. Proponents claim that the curriculum would be standardized across the nation. However, the desire for local control is one of the foundational beliefs of the United States. An individual focus desired by the states would be virtually impossible with national standards. Getting Involved How can you get involved? On an individual level, just learning the state and any national standards will keep you informed of what is current in your field. You should join any organizations for your subject area such as the National Council for Teachers of English (NCTE). This will help you stay up-to-date as national standards are changed. In terms of your individual state, contact the state Department of Education to see if there is a way for you to become involved in reviews and changes to standards. In many states, teachers are selected to be a part of the standards process. In this way, you can have a voice in future changes to the standards for your subject area.
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